Green synthesis of nanoflower-like CuO nanoparticles using lotus leaf extract: Enhanced antibacterial and antioxidant activity

KHAN KADIR; 2; 3; 4

doi:10.62638/ZasMat1691

Authors

Khan Kadir Rafik Department of Chemistry, Sir Sayyed College of Arts, Commerce and Science, Chh. Sambhajinagar (Aurangabad), India Author
Neha Samreen Gulam Dastageer Department of Chemistry, Milind College of Science, Chh. Sambhajinagar (Aurangabad), India Author
Rahul Amrutrao Waghmare Department of Chemistry, Milind College of Science, Chh. Sambhajinagar (Aurangabad), India Author
Momin Nishat Parveen Department of Chemistry, Sir Sayyed College of Arts, Commerce and Science, Chh. Sambhajinagar (Aurangabad), India Author

DOI:

https://doi.org/10.62638/ZasMat1691

Abstract

Copper oxide nanoparticles (CuO NPs) were successfully synthesized via a green route using aquatic lotus (Nelumbonucifera) leaf extract as a reducing and stabilizing agent. The synthesized CuO NPs were characterized using FT-IR, UV–Vis, XRD, SEM, TEM, HR-TEM, SAED, and EDX techniques. FT-IR confirmed Cu–O vibrations at 421 and 560 cm⁻¹, while UV–Vis spectra showed a characteristic absorption peak at 215 nm. XRD analysis revealed a crystalline monoclinic phase with an average crystallite size of 27.88 nm. Electron microscopy confirmed nanoflower-like morphology with an average particle size of ~24.98 nm.The biosynthesized CuO NPs exhibited strong antibacterial activity against Streptococcus pyogenes, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa, with zones of inhibition comparable to the standard antibiotic ciprofloxacin. In addition, CuO NPs demonstrated high antioxidant activity (92.94%), which is comparable to ascorbic acid (92.27%) in DPPH assay. These findings highlight that lotus leaf-mediated CuO nanoparticles are efficient, eco-friendly nanomaterials with promising biomedical applications.

Keywords:

Green approach, Sustainability, Copper Nanoparticles, antimicrobial, antioxidant analysis

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